RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula

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RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula.

RNA interference (RNAi) is a powerful reverse genetic tool to study gene function. The data presented here show that Agrobacterium rhizogenes-mediated RNAi is a fast and effective tool to study genes involved in root biology. The Arabidopsis gene KOJAK, involved in root hair development, was efficiently knocked down. A. rhizogenes-mediated root transformation is a fast method to generate advent...

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Composite Medicago truncatula plants harbouring Agrobacterium rhizogenes-transformed roots reveal normal mycorrhization by Glomus intraradices

Composite plants consisting of a wild-type shoot and a transgenic root are frequently used for functional genomics in legume research. Although transformation of roots using Agrobacterium rhizogenes leads to morphologically normal roots, the question arises as to whether such roots interact with arbuscular mycorrhizal (AM) fungi in the same way as wild-type roots. To address this question, root...

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Alkaloids in Stem Roots of Nicotiana tabacum and Spartium junceum Transformed by Agrobacterium rhizogenes

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ژورنال

عنوان ژورنال: Journal of Experimental Botany

سال: 2004

ISSN: 1460-2431

DOI: 10.1093/jxb/erh122